EVcomplex is a method for predicting inter-protein residue interactons. Two multiple sequence alignments for two protein domains are combined by concatenating sequence pairs, one from each of the domain alignments. Pairing is done by genome coordinates; two sequences in the same genome from nearby locations are most likely to be co-expressed and physically interact. These concatenated pairs are then used as an MSA input into the Evolutionary Couplings method, scoring pairs of residues based on evolutionary co-variation. High scoring ECs are examined to distinguish the inter-ECs (which pair two residues from different domains) from the intra-ECs. The high scoring inter-ECs are likely points of interaction in the interface between the two domains.
This webserver lets you predict EVcouplings between two proteins
- Enter the uniprot identifiers of FASTA sequences of two proteins which are known to interact, and have known bacterial homologs.
- Provide other optional inputs similar to those for EVCoupling
- Get the concatenated pair multiple sequence alignment
- Get the high scoring inter-ECs
- Visualize inter-ECs on known 3D structures of the protein complex (if applicable).